Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Are phosphorylated tyrosine residues of certain receptors involved in inducing transitory covalent protein cross-linking?

Medical hypotheses·1997
Same author

Further studies on targeted DNA transfer to cells using a highly efficient delivery system of biotinylated transferrin and biotinylated polylysine complexed to streptavidin.

Journal of drug targeting·1995
Same author

The history of the Poliomyelitis Research Foundation.

Adler Museum bulletin·1993
Same author

Improved immunoassay for perphenazine.

Clinica chimica acta; international journal of clinical chemistry·1992
Same author

Kawasaki disease manifesting with acute cholangitis. A case report.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·1992
Same author

Immunological disorders--the evolution of an hypothesis.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·1992

Related Experiment Video

Updated: Jun 19, 2026

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
09:21

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Published on: July 20, 2019

A METHOD FOR INTRACELLULAR AUTORADIOGRAPHY IN THE ELECTRON MICROSCOPE.

M H Silk1, A O Hawtrey, I M Spence

  • 1Poliomyelitis Research Foundation, Johannesburg, South Africa.

The Journal of Biophysical and Biochemical Cytology
|October 30, 2009
PubMed
Summary

This study presents a high-resolution electron microscopy technique for intracellular autoradiography. The method visualizes thymidine incorporation in cell cytoplasm, suggesting potential cytoplasmic polynucleotide synthesis.

More Related Videos

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets
10:16

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets

Published on: March 12, 2019

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments
07:51

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Published on: December 21, 2017

Related Experiment Videos

Last Updated: Jun 19, 2026

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
09:21

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Published on: July 20, 2019

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets
10:16

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets

Published on: March 12, 2019

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments
07:51

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Published on: December 21, 2017

Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Molecular Biology

Background:

  • High-resolution intracellular autoradiography is crucial for visualizing molecular processes within cells.
  • Existing techniques may have limitations in resolution or applicability.

Purpose of the Study:

  • To develop and describe a novel technique for high-resolution intracellular autoradiography using electron microscopy.
  • To investigate the site of thymidine incorporation in LLC-MK(2) monkey kidney cells.

Main Methods:

  • LLC-MK(2) cells were labeled with thymidine-H(3) and processed for electron microscopy.
  • A silver metal layer was evaporated onto ultrathin sections, followed by bromine vapor exposure.
  • Autoradiographic exposure was performed, and grids were developed and examined under an electron microscope.

Main Results:

  • The technique yielded well-defined ultrastructural detail.
  • Labeled cells showed an electron-opaque silver deposit in the cytoplasm.
  • This suggests that polynucleotides containing thymidine may be synthesized in the cytoplasm.

Conclusions:

  • The described technique enables high-resolution intracellular autoradiography.
  • Evidence suggests cytoplasmic synthesis of thymidine-containing polynucleotides.
  • This method can be valuable for studying cellular synthesis processes.