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CORRELATION OF THE FINE STRUCTURE OF THE GASTRIC PARIETAL CELL (DOG) WITH FUNCTIONAL ACTIVITY OF THE STOMACH
1Daniel Baugh Institute of Anatomy and the Department of Physiology, Jefferson Medical College of Philadelphia.
The Journal of Biophysical and Biochemical Cytology
|October 30, 2009
Summary
Gastric acid secretion involves changes in parietal (oxyntic) cell structure, specifically in vesicular elements and intracellular canaliculi. These morphological alterations correlate with the cell's role in stomach acid production.
Area of Science:
- Cell biology
- Gastroenterology
- Histology
Background:
- Parietal cells in gastric glands are responsible for acid secretion.
- Understanding their fine structure is key to understanding gastric physiology.
Purpose of the Study:
- To compare the fine structure of dog parietal cells during active acid secretion versus basal states.
- To elucidate the morphological changes associated with gastric acid production.
Main Methods:
- Utilized gastric fistulae in dogs for collecting gastric juice and analyzing acidity.
- Examined mucosal biopsies using electron microscopy after OsO(4) fixation and lead hydroxide staining.
- Stimulated acid secretion via electrical vagal stimulation, histamine, or insulin.
Main Results:
- Observed significant alterations in parietal cell fine structure during acid secretion.
- Changes primarily involved smooth surfaced vesicular elements and intracellular canaliculi.
- Increased concentration of smooth surface profiles near canaliculi and development of prominent microvilli in canaliculi were noted.
- Mitochondria showed some involvement.
Conclusions:
- Morphological changes in parietal cells directly correlate with the acid-secretory state.
- Parietal cells exhibit dynamic structural modifications during gastric acid secretion.
- These findings support the parietal cell's active role in the process of acid secretion.
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