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Related Concept Videos

Techniques for Isolation of Pure Cultures01:24

Techniques for Isolation of Pure Cultures

Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...

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Updated: Jun 19, 2026

Precise, High-throughput Analysis of Bacterial Growth
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Published on: September 19, 2017

A METHOD OF STANDARDIZING BACTERIAL SUSPENSIONS.

F L Gates1

  • 1Laboratories of The Rockefeller Institute for Medical Research.

The Journal of Experimental Medicine
|October 30, 2009
PubMed
Summary
This summary is machine-generated.

Bacterial suspension opacity quantifies bacterial concentration. A simple method and instrument allow for reproducible bacterial concentration measurements, crucial for comparative studies.

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Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient
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Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient

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Last Updated: Jun 19, 2026

Precise, High-throughput Analysis of Bacterial Growth
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Published on: September 19, 2017

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient
05:52

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient

Published on: January 7, 2019

Area of Science:

  • Microbiology
  • Biophysics

Background:

  • Accurate bacterial concentration measurement is vital for microbiological research.
  • Standardization of bacterial suspension opacity measurements is needed.

Purpose of the Study:

  • To develop a standardized method for measuring bacterial suspension opacity.
  • To relate opacity measurements to bacterial concentration (bacteria per cc).

Main Methods:

  • Measuring the length of a bacterial suspension column that obscures a wire loop.
  • Utilizing a simple formula to convert opacity length to bacterial concentration.
  • Describing a novel instrument for opacity measurement.

Main Results:

  • Opacity is directly related to bacterial concentration.
  • The developed method provides comparable bacterial concentration values.
  • A functional instrument for opacity measurement was created.

Conclusions:

  • The described method and instrument enable accurate and reproducible bacterial concentration determination.
  • This technique facilitates standardized comparisons between different bacterial suspensions.