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THE DISTRIBUTION OF CHLORIDES IN THE BLOOD OF THE DOG AFTER EXPERIMENTAL INTESTINAL OBSTRUCTION
The Journal of Experimental Medicine
|October 30, 2009
Summary
Experimental intestinal obstruction in dogs affects body chloride levels. Whole blood chloride measurements accurately reflect total body chloride changes, even when plasma chloride levels are altered.
Area of Science:
- Biochemistry
- Physiology
- Veterinary Medicine
Background:
- Intestinal obstruction can lead to significant physiological disturbances.
- Accurate measurement of electrolytes, such as chloride, is crucial in understanding these disturbances.
Purpose of the Study:
- To present results of simultaneous chloride determinations in canine whole blood, plasma, and cells following experimental intestinal obstruction.
- To compare two methods for estimating chloride content in whole blood and plasma.
Main Methods:
- Simultaneous chloride determinations in whole blood, plasma, and cells.
- Comparison of iodometric titration on protein-free filtrate with the Van Slyke digestion technique for chloride estimation.
Main Results:
- Chloride levels determined by iodometric titration on protein-free filtrate closely matched those from the Van Slyke digestion procedure.
- The iodometric determination accurately reflects blood chloride levels.
- Whole blood chloride measurements best indicate the total change in the body's chloride store.
Conclusions:
- The iodometric titration method is accurate for blood chloride determination.
- Whole blood analysis provides the most comprehensive assessment of chloride shifts during intestinal obstruction.
- Chloride retained in the body during intestinal obstruction is not found in the blood in an identifiable form.
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