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Updated: Jun 19, 2026

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
Published on: October 2, 2017
IMPROVED METHODS FOR THE PREPARATION OF THE SPECIFIC POLYSACCHARIDES OF PNEUMOCOCCUS
M Heidelberger1, C M Macleod, H Markowitz
1Department of Medicine, College of Physicians and Surgeons, Columbia University, the Presbyterian Hospital, and the Department of Microbiology, New York University College of Medicine, New York.
Abstract:
The specific polysaccharides of Types I and V pneumococcus give sharp equivalence zones and show maximal precipitation with homologous rabbit antisera only when carefully prepared from cultures which have not been neutralized with alkali. The precipitating power of S V toward homologous rabbit antiserum falls off in 0.1 N NaOH even more rapidly than that of S I, dropping to 7 per cent of the original value in 6 days at room temperature. The alkaline solution develops a large absorption peak at 270 mmicro. Directions are given for the preparation of S VII and S XII.
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