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06:09
Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
Published on: July 31, 2011
IMMUNOLOGICAL STUDIES ON PEPSIN AND PEPSINOGEN
1Department of Animal and Plant Pathology, and the Laboratories of The Rockefeller Institute, Princeton, N. J.
The Journal of General Physiology
|October 30, 2009
Summary
Pepsin antisera specifically identify pepsin from certain species, distinguishing it from serum proteins. Pepsinogen antisera react with pepsinogen but not with activated pepsin, aiding in enzyme characterization.
Area of Science:
- Biochemistry
- Immunology
- Comparative physiology
Background:
- Pepsin is a key digestive enzyme with varying properties across species.
- Understanding pepsin and pepsinogen antigenicity is crucial for biochemical and immunological studies.
Purpose of the Study:
- To investigate the species-specific antigenic properties of alkali-denatured pepsin.
- To differentiate between pepsinogen and activated pepsin using specific antisera.
Main Methods:
- Preparation of antisera against pepsin and pepsinogen from various species.
- Immunoprecipitation assays to test cross-reactivity between denatured pepsins and antisera.
- Analysis of reactions between pepsinogen, activated pepsin, and homologous antisera.
Main Results:
- Alkali-denatured pepsin from swine, cattle, and guinea pigs precipitated with swine pepsin antiserum, unlike rabbit, chicken, and shark pepsin.
- Pepsin antisera cross-reacted with both pepsin and pepsinogen but not with homologous serum proteins.
- Pepsinogen antisera reacted with pepsinogen, including activated forms, but not with purified pepsin or serum proteins.
Conclusions:
- Pepsin antigenicity exhibits species-specific characteristics, particularly after alkali denaturation.
- Pepsin and pepsinogen share antigenic determinants, but pepsinogen antisera can distinguish between the zymogen and the active enzyme.
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