Related Experiment Video
Updated: Jun 19, 2026

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
Published on: June 21, 2021
SOME FACTORS WHICH INFLUENCE THE OXIDATION OF SULFHYDRYL GROUPS
1Laboratories of The Rockefeller Institute for Medical Research, Princeton, New Jersey.
Cyanide inhibits SH group oxidation by the uric acid reagent. Copper sulfate addition enables cysteine oxidation by uric acid and ferricyanide reagents, impacting protein chemistry research.
Area of Science:
- Biochemistry
- Protein Chemistry
Background:
- Sulfhydryl (SH) groups in proteins are crucial for structure and function.
- Understanding the factors influencing SH group reactivity is vital for biochemical research.
Purpose of the Study:
- To investigate the oxidation of sulfhydryl groups in cysteine and egg albumin.
- To determine the effects of various reagents and conditions on SH group oxidation.
Main Methods:
- Utilized uric acid reagent and potassium ferricyanide for oxidation.
- Examined reactions in different pH conditions (acidic, neutral).
- Assessed the influence of denaturing agents (urea, alkyl sulfate, guanidine hydrochloride) and copper sulfate.
Main Results:
- Cyanide inhibited SH group oxidation by the uric acid reagent.
- Copper sulfate was essential for cysteine oxidation by both reagents at pH 4.8.
- The uric acid reagent's efficacy depended on denaturing agents and protein hydrolysis.
- Ferricyanide demonstrated broader efficacy in oxidizing SH groups, even under non-denaturing conditions with precautions.
Conclusions:
- Reagent choice, pH, denaturing agents, and protein modification significantly affect SH group oxidation.
- Copper ions play a critical role in facilitating SH group oxidation.
- Findings provide insights into the chemical reactivity of sulfhydryl groups in biological molecules.
More Related Videos
09:37Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
Published on: May 2, 2019
03:35Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
Published on: June 28, 2024
Related Concept Videos
Preparation and Reactions of Thiols
Preparation and Reactions of Sulfides
Oxidation of Phenols to Quinones
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox property is crucial in...
Structure and Nomenclature of Thiols and Sulfides
Sulfur Assimilation
Oxidations of Aldehydes and Ketones to Carboxylic Acids
Aldehydes readily undergo oxidation in strong oxidizing agents such as potassium permanganate and chromic acid. The oxidation can also be carried out using mild oxidizing agents such as silver oxide. In fact, aldehydes can be easily oxidized...