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Binding proteins from animals with possible transport function.

R H Wasserman1, R A Corradino, A N Taylor

  • 1Department of Physical Biology, New York State Veterinary College, Cornell University, Ithaca, New York 14850.

The Journal of General Physiology
|October 30, 2009
PubMed
Summary
This summary is machine-generated.

This study identifies a vitamin D-induced calcium-binding protein (CaBP) crucial for calcium absorption in the small intestine. Its concentration correlates with calcium uptake, suggesting a key transport role.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Physiology

Background:

  • Proteins with transport functions are vital in various animal tissues.
  • Vitamin D influences calcium metabolism and absorption.
  • Calcium translocation across epithelial membranes is a key physiological process.

Purpose of the Study:

  • To describe vitamin D-induced calcium-binding protein (CaBP) and its role in calcium translocation.
  • To investigate the presence and properties of CaBP in different animal species and tissues.
  • To elucidate the involvement of CaBP in intestinal calcium absorption.

Main Methods:

  • Isolation and purification of CaBP from chick intestinal mucosa.
  • Determination of CaBP properties including molecular weight, formation constant, and binding capacity.
  • Correlative studies on CaBP concentration and calcium absorptive capacity in the gut.
  • Immunolocalization of CaBP within intestinal absorptive cells.

Main Results:

  • CaBP was identified in the small intestine, colon, kidney, and uterus (shell gland) of chickens, and in the small intestine of rats, dogs, bovines, and monkeys.
  • Purified chick intestinal CaBP has a molecular weight of approximately 28,000, a formation constant of 2.6 x 10(5) M(-1), and binds one calcium atom per molecule.
  • CaBP concentration in intestinal mucosa directly correlates with the gut's calcium absorptive capacity.
  • CaBP was localized to the brush border region of intestinal absorptive cells and goblet cells.

Conclusions:

  • CaBP is intimately involved in the process of calcium absorption across epithelial membranes.
  • The presence and concentration of CaBP are indicative of its critical role in calcium transport.
  • CaBP represents a significant molecular player in vitamin D-mediated calcium homeostasis.