[Study on refining process of Flos Chrysanthemi extract with macroporous resin]

Area of Science:

• Phytochemistry
• Natural Product Chemistry
• Separation Science

Context:

• Flos Chrysanthemi (Chrysanthemum flower) is a traditional herbal medicine with valuable bioactive compounds.
• Efficient extraction and purification of these compounds are crucial for pharmaceutical and nutraceutical applications.
• Macroporous adsorption resins offer a promising method for selective extraction and purification of plant-derived compounds.

Purpose:

• To determine optimal parameters for refining Flos Chrysanthemi extract using macroporous adsorption resin.
• To investigate the effects of different resin types, liquor volumes, and elution solvents on the extraction efficiency.
• To validate the purification process by assessing the purity of key active components: chlorogenic acid, total flavonoids, and luteolin.

Summary:

• Macroporous resin F was identified as optimal for Flos Chrysanthemi extract refinement.
• The maximum adsorption capacity for chlorogenic acid was 10.8 mg/g dry resin.
• An elution solvent of 5-fold column volume of 80% alcohol yielded high purity for total flavonoids (589.2%), chlorogenic acid (605.4%), and luteolin (650.1%).

Impact:

• The optimized process significantly enhances the purity of active compounds from Flos Chrysanthemi.
• This method provides an effective strategy for the industrial-scale purification of valuable phytochemicals.
• The findings contribute to the development of standardized herbal extracts with improved therapeutic potential.