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Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

High-dynamic-range quantitative phase imaging with spectral domain phase microscopy.

Jun Zhang¹, Bin Rao, Lingfeng Yu

¹Beckman Laser Institute and the Center for Biomedical Engineering, University of California, Irvine, Irvine, California 92612, USA. junzhang@uci.edu

|November 3, 2009

Summary

This summary is machine-generated.

High-dynamic-range phase microscopy achieves sensitive displacement measurements using Fourier domain optical coherence tomography. This advanced technique overcomes limitations for detailed imaging of transparent objects and biological cells.

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Area of Science:

Biomedical Optics
Microscopy
Cell Biology

Background:

Quantitative phase imaging offers label-free visualization of transparent specimens.
Traditional phase microscopy faces limitations in dynamic range and sensitivity.
Optical coherence tomography (OCT) provides high-resolution cross-sectional imaging.

Purpose of the Study:

To demonstrate high-dynamic-range quantitative phase-contrast imaging using a novel microscopy technique.
To achieve highly sensitive displacement measurements for transparent phase objects.
To evaluate the system's performance for biological cell imaging.

Main Methods:

Implementation of a common-path Fourier domain optical coherence tomography (FDOCT) system.
Application of a phase retrieval approach in the spectral domain to overcome 2pi ambiguity.
Utilizing phase microscopy for quantitative phase-contrast imaging.

Main Results:

Demonstrated high-dynamic-range quantitative phase imaging of transparent phase objects.
Achieved displacement measurement sensitivity of 34 picometers (pm).
Successfully performed two-dimensional quantitative phase imaging of human neonatal dermal keratinocyte cells.

Conclusions:

The developed phase microscopy technique enables high-dynamic-range quantitative phase imaging.
The system offers exceptional sensitivity for displacement measurements, overcoming previous limitations.
The technique is suitable for detailed imaging and analysis of biological cells.