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C-reactive protein: how conformational changes influence inflammatory properties.

Steffen U Eisenhardt¹, Jan R Thiele, Holger Bannasch

¹Department of Plastic and Hand Surgery, University of Freiburg Medical Centre, Freiburg, Germany. steffen.eisenhardt@uniklinik-freiburg.de

Cell Cycle (Georgetown, Tex.)

|November 6, 2009

Summary

C-reactive protein (CRP) exists in two forms: pentameric (pCRP) and monomeric (mCRP). Dissociation of pCRP to mCRP, observed in inflamed tissues, reveals mCRP as a pro-inflammatory agent, suggesting it as a therapeutic target.

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Area of Science:

Biochemistry
Immunology
Molecular Biology

Background:

C-reactive protein (CRP) is a key acute phase reactant.
Evidence suggests CRP contributes to inflammatory diseases.
CRP exists as pentameric (pCRP) and monomeric (mCRP) isoforms with distinct inflammatory profiles.

Purpose of the Study:

To review the literature on the causal role of pCRP and mCRP in inflammatory diseases.
To analyze current controversies surrounding CRP's function.
To highlight mCRP and pCRP dissociation as potential therapeutic targets.

Main Methods:

Literature review of CRP's role in inflammation.
Analysis of pCRP dissociation mechanisms.
In vitro studies on CRP isoform inflammatory profiles.

Main Results:

Identified localized pCRP dissociation by activated platelets and apoptotic cells.
Showed mCRP deposition in inflamed tissues.
Demonstrated distinct pro-inflammatory profiles for mCRP versus pCRP.

Conclusions:

Localized pCRP dissociation generates pro-inflammatory mCRP.
mCRP plays a causal role in inflammatory reactions.
Targeting mCRP and the pCRP dissociation process offers a novel therapeutic strategy.