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Related Experiment Video

Updated: Jun 18, 2026

Tracking Mouse Bone Marrow Monocytes In Vivo
12:08

Tracking Mouse Bone Marrow Monocytes In Vivo

Published on: February 27, 2015

Measuring bone blood supply in mice using fluorescent microspheres.

Maria A Serrat1

  • 1Department of Biomedical Sciences, Kent State University, Kent Ohio, USA. maria.a.serrat@gmail.com

Nature Protocols
|November 7, 2009
PubMed
Summary

This study presents a modified fluorescent microsphere technique for measuring bone and organ blood flow in mice. This method offers a viable alternative for small mammal research where traditional techniques are challenging.

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Area of Science:

  • Physiology
  • Biomedical Engineering
  • Animal Models

Background:

  • Fluorescent microspheres are standard for assessing bone blood supply in large animals.
  • Traditional methods for measuring perfusion are difficult to apply in small mammals like mice.
  • A need exists for adaptable techniques to study microcirculation in small animal models.

Purpose of the Study:

  • To describe a modified fluorescent microsphere protocol for quantifying bone and organ perfusion in mice.
  • To provide a practical alternative to existing methods for small mammal research.
  • To establish a reliable method for assessing relative blood supply in mouse tissues.

Main Methods:

  • Injection of fluorescent microspheres directly into the left ventricle of the heart.
  • Utilizing a reference tissue to quantify microsphere deposition.
  • Tissue processing including decalcification and digestion for microsphere analysis.

Main Results:

  • The protocol enables measurement of relative bone and organ blood supply in mice.
  • The entire procedure, including pauses, takes 140.5 hours for 15 mice (5 tissues each).
  • The bench time required, excluding pauses, is 20.5 hours.

Conclusions:

  • Modified fluorescent microsphere technique is a viable method for assessing bone and organ perfusion in mice.
  • This protocol overcomes limitations of traditional methods in small animal research.
  • The technique provides a valuable tool for studying microvascular function in mouse models.