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Archetype JC virus efficiently propagates in kidney-derived cells stably expressing HIV-1 Tat

Souichi Nukuzuma1, Masanori Kameoka, Shigeki Sugiura

  • 1Department of Microbiology, Kobe Institute of Health, 4-6, Minatojima-Nakamachi, Chuo-ku, Kobe, Hyogo 650-0046, Japan. s-nuku@gj8.so-net.ne.jp

Microbiology and Immunology
|November 12, 2009
PubMed

Insights

HIV-1 Tat protein significantly increases archetype John Cunningham virus (JCV) replication in kidney cells. This finding suggests Tat

Area of Science:

  • Virology
  • Neuroscience
  • Immunology

Background:

  • Progressive multifocal leukoencephalopathy (PML) is caused by pathogenic JCV with rearranged regulatory regions.
  • Archetype JCV, typically found in the kidneys, may convert to the PML-type under immunosuppression.
  • HIV-1 Tat protein is known to enhance JCV late promoter activity.

Purpose of the Study:

  • To investigate the influence of HIV-1 Tat on archetype JCV propagation.
  • To establish a cell culture system for studying Tat's role in JCV replication.

Main Methods:

  • Utilized kidney-derived COS-7 cells permitting archetype JCV replication.
  • Established COS-tat cells stably expressing HIV-1 Tat.
  • Compared archetype JCV propagation in COS-7 and COS-tat cells.

Main Results:

  • Archetype JCV propagation was significantly greater in COS-tat cells compared to COS-7 cells.
  • HIV-1 Tat expression directly correlated with increased archetype JCV production.
  • SV40 T antigen expression, though slightly enhanced by Tat, was not the primary driver of increased JCV replication.

Conclusions:

  • COS-tat cells provide a novel system for studying Tat's role in archetype JCV replication.
  • This research offers insights into the development of PML in HIV-1-infected individuals.
  • The efficiency of JCV propagation is dependent on functional Tat expression.

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