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Updated: Jun 18, 2026
Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
Published on: February 23, 2020
Towards an electrowetting-based digital microfluidic platform for magnetic immunoassays
Vincent Schaller1, Anke Sanz-Velasco, Alexey Kalabukhov
1Department of Microtechnology and Nanoscience-MC2, Chalmers University of Technology, SE-412 96, Göteborg, Sweden. vincent.schaller@chalmers.se
Abstract:
We demonstrate ElectroWetting-On-Dielectric (EWOD) transport and SQUID gradiometer detection of magnetic nanoparticles (MNPs) suspended in a 2 microl de-ionized water droplet. This proof-of-concept methodology constitutes the first development step towards a highly sensitive magnetic immunoassay platform with SQUID readout and droplet-based sample handling. Magnetic AC-susceptibility measurements were performed on MNPs with a hydrodynamic diameter of 100 nm using a high-Tc dc Superconducting Quantum Interference Device (SQUID) gradiometer as detector. We observed that the signal amplitude per unit volume is 2.5 times higher for a 2 microl sample droplet compared to a 30 microl sample volume.
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