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Updated: Jun 18, 2026

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
Published on: January 7, 2019
Contribution of two approaches using electrospray ionization with multi-stage mass spectrometry for the
Corinne Loutelier-Bourhis1, Ornella Zovi, Laurence Lecamp
1Université de Rouen, CNRS UMR 6014, COBRA, Rue Tesnière, 76821 Mont Saint Aignan Cedex, France. Corinne.Loutelier@univ-rouen.fr
Abstract:
A detailed characterization of triacylglycerols (TAGs) present in linseed oil samples from a local producer was performed using electrospray ionization and two mass spectrometric approaches; direct infusion multi-stage mass spectrometry (MS(n)) experiments and liquid chromatography/tandem mass spectrometry (LC/MS/MS) using non-aqueous reversed-phase chromatographic conditions. The combination of both approaches permitted the identification of 26 TAGs. Comparison of the two analytical approaches showed that discrimination of regioisomers was achieved from MS3 data while other isobaric species were separated and identified by LC/MS/MS analysis. The results we obtained were also compared with those previously reported. The TAG composition of the studied linseed oil is qualitatively identical to that of linseed oils from various sources in Europe, Canada, Argentina or India. However, a few differences were observed with regard to the proportions of some TAGs; these can be explained by variations in the culture conditions, climate, and variety of the seeds.
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