Improved specific biodetection with ion trap mobility spectrometry (ITMS): a 10-min, multiplexed, immunomagnetic
Andrew D Pris1, Frank J Mondello, Ronald J Wroczynski
1General Electric-Global Research Center, Niskayuna, New York 12309, USA. pris@research.ge.com
Analytical Chemistry
|November 18, 2009
Summary
A new immunomagnetic enzyme-linked immunosorbent assay (ELISA) was developed for rapid, field-deployable trace chemical detection. This optimized assay provides quantitative results in 10 minutes using a commercial ion trap mobility spectrometer.
Area of Science:
- Analytical Chemistry
- Biotechnology
Background:
- Field-deployable trace chemical detection equipment is needed for logistical, training, and maintenance benefits.
- Integrating biodetection assays with existing equipment simplifies field operations.
Purpose of the Study:
- To develop and optimize an immunomagnetic enzyme-linked immunosorbent assay (ELISA) for analysis by a commercial off-the-shelf (COTS) ion trap mobility spectrometer.
- To enable rapid, quantitative detection of biological targets in field settings.
Main Methods:
- An immunomagnetic ELISA was designed using undetectable enzymatic substrates and detectable ELISA-generated products.
- The assay was optimized for quantitative analysis using an unmodified ion trap mobility spectrometer.
- New substrate/enzyme/product combinations for the immunomagnetic ELISA were explored.
Main Results:
- The optimized ELISA retained assay specificity and a detection limit of approximately 10^3 E. coli per assay.
- Assay time was reduced to 10 minutes, a >9-fold decrease compared to previous studies.
- The number of user steps was significantly reduced.
Conclusions:
- A rapid, quantitative, 10-minute immunomagnetic ELISA analyzed by an ion trap mobility spectrometer is feasible.
- This approach simplifies field biodetection logistics and training.
- The discovered substrate/enzyme/product combinations enable potential for quantitative, multiplexed assays.
