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Updated: Jun 18, 2026

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Published on: November 11, 2018
Rab35--a vesicular traffic-regulating small GTPase with actin modulating roles
Christelle En Lin Chua1, Yi Shan Lim, Bor Luen Tang
1Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
Rab35, a Rab GTPase, influences cell shape by regulating actin dynamics. It achieves this by activating Rho GTPases or interacting with the actin-bundling protein fascin.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Rab GTPases regulate vesicular transport, while Rho GTPases control actin dynamics.
- Rab35 localizes to plasma membranes and endosomes, participating in processes like T-cell receptor recycling and cytokinesis.
- Rab35's role in neurite outgrowth and cell protrusions suggests a link to cytoskeletal regulation.
Purpose of the Study:
- To investigate the mechanism by which Rab35 influences actin dynamics and cell protrusions.
- To determine if Rab35 directly impacts actin cytoskeleton organization.
Main Methods:
- Utilized cell-based assays to observe Rab35-mediated effects on cell morphology.
- Investigated the molecular pathways involved in Rab35-induced actin rearrangements.
Main Results:
- Rab35 directly modulates actin dynamics, leading to the formation of cell protrusions.
- Rab35's protrusive activity is mediated through either Rho GTPase activation or interaction with the actin-bundling protein fascin.
Conclusions:
- Rab35 possesses a dual role in regulating vesicular traffic and actin dynamics.
- This study reveals Rab35 as a key regulator connecting membrane trafficking and cytoskeletal organization.
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