Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spot on: A Laser Micromachining-Based Approach to Improve Dried Matrix Spot Preparation with Proof-of-Principle Analytical Demonstrations Using Ambient Ionization Mass Spectrometry.

Micromachines·2026
Same author

Reverse-Flow Engineering of a Liquid Microjunction Surface Sampling Probe for Ambient Ionization Mass Spectrometry Using Computational Fluid Dynamics.

Analytical chemistry·2026
Same author

Rapid Screening and Prioritization of Culture Conditions for Natural Product Discovery using the Liquid Microjunction Surface Sampling Probe.

Journal of the American Society for Mass Spectrometry·2025
Same author

Microarray-Based Nanoextraction Combined with Ambient Ionization Mass Spectrometry for Analysis of Drugs of Abuse in Wastewater.

Analytical chemistry·2025
Same author

Activation of Primary C-H Bonds in Oxidative Cyclizations of Tambjamines Catalyzed by Rieske Oxygenases TamC and <i>Pt</i>TamC.

Journal of the American Chemical Society·2025
Same author

Rapid and Robust Workflows Using Different Ionization, Computation, and Visualization Approaches for Spatial Metabolome Profiling of Microbial Natural Products in <i>Pseudoalteromonas</i>.

ACS measurement science au·2024
Same journal

An Overview of Pterin Analysis in Biological Samples: From Occurrence and Properties to Sample Pretreatment Combined With Hyphenated Separation Techniques.

Journal of separation science·2026
Same journal

Chromatographic Purification of Complex Natural Products as a Decision Problem: Retention Prediction, Adaptive Optimization, and Experimental Feedback.

Journal of separation science·2026
Same journal

A High-Throughput Analytical Approach Using Polyaniline Doped With Oxalic Acid in Thin Film Solid-Phase Microextraction for the Determination of Personal Care Products in Recreational Waters.

Journal of separation science·2026
Same journal

Simultaneous Determination of Dechlorane-Related Compounds in Fish Muscle by Microwave-Assisted Extraction Combined With Enhanced Matrix Removal for Lipids Cleanup and Gas Chromatography-Tandem Mass Spectrometry.

Journal of separation science·2026
Same journal

Covalent Zwitterionic Peptide-Based Antifouling Coating of the Fused Silica Capillary Applied for CE Separation of Proteins.

Journal of separation science·2026
Same journal

Pharmacokinetic Study of Five Lead Components of Psoraleae Fructus in Human Serum by UHPLC-Q-TOF-MS and UHPLC-QqQ-MS/MS after Oral Administration of Qing'e Pills.

Journal of separation science·2026
See all related articles

Related Experiment Video

Updated: Jun 18, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
13:36

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Published on: December 4, 2021

Reversed phase capillary HPLC using polymer-entrapped C18 particles.

Graham T T Gibson1, Dale M Marecak, Richard D Oleschuk

  • 1Department of Chemistry, Queen's University, Kingston, Ontario, Canada.

Journal of Separation Science
|November 26, 2009
PubMed
Summary
This summary is machine-generated.

New capillary columns use polymer-entrapped silica microspheres for chromatography. These fritless columns offer high pressure stability and potential for mass production, showing performance comparable to commercial options for LC-MS applications.

More Related Videos

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

Related Experiment Videos

Last Updated: Jun 18, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
13:36

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Published on: December 4, 2021

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Materials Science

Background:

  • Traditional capillary columns often require frits, which can be a limitation in manufacturing and performance.
  • Octadecylsilyl-modified silica microspheres are widely used stationary phases in chromatography.
  • Polymer entrapment offers a novel method for immobilizing chromatographic particles.

Purpose of the Study:

  • To evaluate the chromatographic performance of novel capillary columns packed with polymer-entrapped octadecylsilyl-modified silica microspheres.
  • To assess the stability and manufacturing potential of these fritless columns.
  • To explore their suitability for capillary liquid chromatography-mass spectrometry (LC-MS).

Main Methods:

  • Photoinitiated polymerization was used to entrap octadecylsilyl-modified silica microspheres within capillary columns.
  • Various entrapment styles were investigated.
  • Chromatographic performance was evaluated, and pressure stability was tested up to 5000 psi (345 bar).

Main Results:

  • The polymer formed at the particle surface, immobilizing microspheres within seconds without needing a frit.
  • All tested entrapment configurations demonstrated high pressure resistance (≥5000 psi).
  • Columns with minimal entrapped regions exhibited performance equivalent to commercial columns.

Conclusions:

  • The developed fritless capillary columns offer a promising alternative for chromatographic separations.
  • The photoinitiated polymerization and fritless design enable mass production capabilities.
  • These columns hold significant potential for applications in capillary LC-MS due to their performance and emitter capabilities.