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Exploring the Potential of Mesenchymal Stem Cell Sheet on The Development of Hepatocellular Carcinoma In Vivo
Published on: September 11, 2018
Multilineage potential of bone-marrow-derived mesenchymal stem cell cell sheets: implications for tissue engineering
Eugene Yong-Shun See1, Siew Lok Toh, James Cho Hong Goh
1National University of Singapore, Singapore, Singapore.
Tissue Engineering. Part A
|December 3, 2009
Summary
Bone-marrow-derived mesenchymal stem cells (BMSCs) form viable cell sheets retaining multipotency. This cell sheet technology offers a promising alternative for tissue engineering applications.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Cell Biology
Background:
- Bone-marrow-derived mesenchymal stem cells (BMSCs) possess multipotent differentiation potential, making them valuable for tissue engineering.
- Cell sheet technology, involving culturing cells to hyperconfluence, is emerging as an efficient method to reduce cell loss during scaffold seeding.
Purpose of the Study:
- To characterize the formation of BMSC cell sheets under hyperconfluent conditions.
- To evaluate the impact of hyperconfluent culture on the multipotentiality of BMSCs.
Main Methods:
- Culture of BMSCs to achieve hyperconfluence and form cell sheets.
- Assessment of BMSC cell sheet viability and composition (Type I collagen).
- Verification of retained multipotency of BMSCs within the cell sheets.
Main Results:
- BMSC cell sheets were successfully formed and maintained viability.
- The cell sheets were found to be rich in Type I collagen.
- BMSCs within the cell sheets demonstrated retained multipotential differentiation capacity.
Conclusions:
- BMSC cell sheets can be formed while preserving cell viability and multipotency.
- This cell sheet approach represents a promising advancement over conventional single-cell suspension seeding for tissue engineering.
- Further applications of BMSC cell sheets in tissue engineering are warranted.
