Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

<i>In Vivo</i> Platelet Detection Using a Glycoprotein IIb/IIIa-Targeted Near-Infrared Fluorescence Imaging Probe.

ACS sensors·2021

Same author

Blood Accessibility to Fibrin in Venous Thrombosis is Thrombus Age-Dependent and Predicts Fibrinolytic Efficacy: An In Vivo Fibrin Molecular Imaging Study.

Theranostics·2015

Same author

Versatile click alginate hydrogels crosslinked via tetrazine-norbornene chemistry.

Biomaterials·2015

Same author

Site-specific labeling of proteins and peptides with trans-cyclooctene containing handles capable of tetrazine ligation.

Chemical biology & drug design·2014

Same author

BODIPY-tetrazine derivatives as superbright bioorthogonal turn-on probes.

Angewandte Chemie (International ed. in English)·2013

Same author

Upconverting organic dye doped core-shell nano-composites for dual-modality NIR imaging and photo-thermal therapy.

Theranostics·2013

Search research articles

Related Experiment Video

Updated: Jun 18, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Labels and probes for live cell imaging: overview and selection guide.

Scott A Hilderbrand¹

¹Center for Molecular Imaging Research, Massachusetts General Hospital/Harvard Medical School, Charlestown, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|December 4, 2009

Summary

Selecting the right fluorescent imaging agents for molecular biology is challenging. This report overviews available probes and their properties to aid researchers in choosing optimal tools for live-cell imaging.

More Related Videos

Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics

Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics

Published on: March 18, 2021

Using LEXY and LINuS Optogenetics Tools and Automated Image Analysis to Quantify Nucleocytoplasmic Transport Dynamics in Live Cells

Using LEXY and LINuS Optogenetics Tools and Automated Image Analysis to Quantify Nucleocytoplasmic Transport Dynamics in Live Cells

Published on: July 22, 2025

Related Experiment Videos

Last Updated: Jun 18, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics

Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics

Published on: March 18, 2021

Using LEXY and LINuS Optogenetics Tools and Automated Image Analysis to Quantify Nucleocytoplasmic Transport Dynamics in Live Cells

Using LEXY and LINuS Optogenetics Tools and Automated Image Analysis to Quantify Nucleocytoplasmic Transport Dynamics in Live Cells

Published on: July 22, 2025

Area of Science:

Molecular Biology
Biochemistry
Cell Biology

Background:

Fluorescence imaging is crucial for studying molecular processes in living cells.
A vast selection of fluorescent labels and activatable probes exist for molecular investigations.
Choosing the correct imaging agent is complex due to numerous options and variables.

Purpose of the Study:

To provide an overview of fluorescent imaging agents.
To detail the optical and physical properties of these agents.
To assist researchers in selecting appropriate probes for their experiments.

Main Methods:

Literature review of fluorescent imaging agents.
Analysis of optical properties (e.g., excitation/emission spectra, quantum yield).
Evaluation of physical properties (e.g., solubility, photostability).

Main Results:

Categorization of various fluorescent probes.
Compilation of key optical and physical characteristics for each probe type.
Discussion of how these properties influence imaging performance.

Conclusions:

Understanding probe properties is essential for successful fluorescence imaging.
This overview serves as a guide for selecting optimal probes.
Proper probe selection enhances the investigation of biochemical processes in living cells.