Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deep proteome coverage advances knowledge of Treponema pallidum protein expression profiles during infection.

Scientific reports·2023
Same author

Universal method for the isolation of microvessels from frozen brain tissue: A proof-of-concept multiomic investigation of the neurovasculature.

Brain, behavior, & immunity - health·2023
Same author

Evolution of biomarker research in autoimmunity conditions for health professionals and clinical practice.

Progress in molecular biology and translational science·2022
Same author

Proteomic Portraits Reveal Evolutionarily Conserved and Divergent Responses to Spinal Cord Injury.

Molecular & cellular proteomics : MCP·2021
Same author

Mass Spectrometric Determination of Protein Ubiquitination.

Methods in molecular biology (Clifton, N.J.)·2019
Same author

Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments.

Nature methods·2019
Same journal

UHPLC-HR-IDX-Orbitrap-MS Profiling of Eryngium triquetrum Vahl: Chemical Characterization and Associated Biological Activities.

Journal of mass spectrometry : JMS·2026
Same journal

Helium Saver Mode With Nitrogen Make-Up Cooling Gas for GC-MS With Cold EI.

Journal of mass spectrometry : JMS·2026
Same journal

Petroleomics FT-ICR MS Data Processing Methods for Inter-Basin Discrimination of Lacustrine Oils From Two Brazilian Pre-Salt Basins.

Journal of mass spectrometry : JMS·2026
Same journal

In Memory of Prof. Günter Allmaier (1956-2022).

Journal of mass spectrometry : JMS·2026
Same journal

A Step-by-Step Protocol From METASPACE to Biological Interpretation.

Journal of mass spectrometry : JMS·2026
Same journal

Rapid Determination of 13 Insecticides and Acaricides in Human Blood Using Dispersive Liquid-Liquid Microextraction Coupled With DART-MS/MS.

Journal of mass spectrometry : JMS·2026
See all related articles

Related Experiment Video

Updated: Jun 18, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Current trends in quantitative proteomics.

Monica H Elliott1, Derek S Smith, Carol E Parker

  • 1University of Victoria Genome BC Proteomics Centre, British Columbia, V8Z 7X8, Canada.

Journal of Mass Spectrometry : JMS
|December 4, 2009
PubMed
Summary
This summary is machine-generated.

Quantifying proteins using mass spectrometry is challenging. This review explores various relative and absolute protein quantitation techniques, offering guidance for researchers to select the best method for their specific biological questions.

More Related Videos

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
05:37

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

Published on: October 20, 2020

Related Experiment Videos

Last Updated: Jun 18, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
05:37

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

Published on: October 20, 2020

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Mass spectrometry (MS) has enabled protein identification in biological samples.
  • The subsequent challenge is to quantify protein abundance accurately.
  • Protein quantitation is crucial for understanding biological processes and disease mechanisms.

Purpose of the Study:

  • To review diverse mass spectrometry-based methods for protein quantitation.
  • To provide guidance for selecting appropriate quantitative techniques for specific research needs.
  • To highlight the dynamic nature of quantitative proteomics research.

Main Methods:

  • Discussion of relative quantitation strategies using mass spectrometry.
  • Exploration of absolute quantitation approaches with mass spectrometry.
  • Overview of various mass spectrometry techniques applied to protein quantification.

Main Results:

  • No single mass spectrometry method is universally applicable for all protein quantitation tasks.
  • A range of techniques exists for both relative and absolute protein quantification.
  • Current methods provide valuable data on biomarkers and biological pathways.

Conclusions:

  • Selecting the optimal mass spectrometry technique depends on the specific biological question and sample.
  • The field of quantitative proteomics is rapidly evolving with new methods and discoveries.
  • Despite limitations, existing mass spectrometry techniques offer significant insights into protein abundance and function.