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Published on: April 18, 2019
Authentication and banking of human pluripotent stem cells
Richard Josephson1, Jonathan Auerbach
1GlobalStem, Rockville, Maryland, USA.
Current Protocols in Stem Cell Biology
|December 5, 2009
Summary
Properly banking and authenticating pluripotent stem cell lines ensures their preservation and reproducibility. Implementing basic guidelines protects valuable research data and cell stocks for future use.
Area of Science:
- Stem cell biology
- Cellular and molecular medicine
Background:
- Pluripotent human stem cell lines exhibit significant variability in differentiation potential, genomic stability, and immunophenotype.
- Differences among cell lines impact research reproducibility and necessitate standardized preservation methods.
Purpose of the Study:
- To provide basic guidelines for the banking and authentication of pluripotent stem cell lines.
- To ensure the long-term preservation and experimental reproducibility of valuable stem cell stocks.
Main Methods:
- Cell banking involves disciplined preservation of cell stocks in their original or baseline state.
- Authentication verifies unique cell line identity, sterility, genomic integrity, stem cell marker expression, and pluripotency upon differentiation.
Main Results:
- A range of assays are available to verify each of the five essential properties of pluripotent stem cell lines.
- Recent advances in molecular biology and service providers have made banking and authentication cost-effective.
Conclusions:
- Implementing standardized banking and authentication protocols is crucial for maintaining the integrity of pluripotent stem cell lines.
- These practices safeguard research data and enable future reproducibility, despite inherent cell line variability.
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