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Utilizing the third order advantage with isotope dilution mass spectrometry
Analytical Chemistry
|December 8, 2009
Summary
A novel isotope dilution mass spectrometry method uses (13)C labeled standards for accurate analyte quantification. Two-dimensional gas chromatography and advanced data analysis isolate and resolve analyte peaks for precise concentration measurements.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Chromatography
Background:
- Isotope dilution mass spectrometry (IDMS) is a gold standard for accurate quantification.
- Traditional methods can be complex, requiring multiple steps or injections.
- The need for robust, single-injection quantification methods persists.
Discussion:
- This study presents a single-injection IDMS method using (13)C labeled analytes.
- Two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GC x GC-TOFMS) generates a third-order data cube.
- Parallel factor analysis (PARAFAC) isolates the combined (12)C and (13)C analyte peak, leveraging the 'third order advantage' against noise and interferences.
Key Insights:
- Classical least squares (CLS) resolves the combined mass spectra to determine the (12)C/(13)C ratio.
- This ratio allows for the absolute quantification of both (12)C and (13)C isotopes.
- Excellent agreement was observed between prepared and determined concentration ratios in test analytes.
Outlook:
- The method demonstrates applicability to real-world samples, highlighting its practical utility.
- This approach offers a robust alternative to standard addition for accurate analyte quantification.
- Further applications in complex matrices and trace analysis are anticipated.
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