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Updated: Jun 18, 2026
Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Published on: February 25, 2010
[Expression and analysis of recombinant chicken IL-18 in Pichia pastoris.]
Min Zhao1, Chun-Jie Zhang, Yin-Ju Li
1Animal Science and Technology College, Henan University of Science and Technology, Luoyang 471003, China.
Aim:
Expression and analysis of recombinant chicken IL-18 in Pichia pastoris.
Methods:
Chicken IL-18 mature peptide gene was amplified from the recombinant plasmid pMD18-T-ChIL-18 by PCR, and was subcloned into Pichia pastoris expression vector pPICZalphaA to construct the recombinant plasmid pPICZalphaA-ChIL-18. After identified by restriction enzymes digestion analysis, PCR and DNA sequencing, the recombinant plasmid was transformed into Pichia pastoris X-33.Then choosing the multi-copy recombinant strains to be induced for expression.Then the bioactivity of rchIL-18 was analysed by Western blot, ELISA and MTT after purified by Sephadex G-100 column.
Results:
The chicken IL-18 with the immunogenicity was secreted by Pichia pastoris. It could induce T lymphocytes proliferation and secreting IFN-gamma in vitro.
Conclusion:
The chicken IL-18 with obvious biological activity is secreted by Pichia pastoris X-33.
