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Published on: November 2, 2013
[Human hnRNP I prokaryotic expression and preliminary application.].
Cheng-Tao Sun1, Fan Feng, Lei Wang
1Department of Immunology, Weifang Medical University; Key Laboratory of Immunology in Universities of Shandong, Weifang 261042, China.
Summary
Researchers successfully expressed recombinant human heterogeneous nuclear ribonucleoprotein I (hnRNP I) protein. This protein showed a high positive ratio for autoantibodies in systemic sclerosis (SSc) patients, indicating its diagnostic potential.
Area of Science:
- Molecular Biology
- Immunology
- Rheumatology
Background:
- Autoantibodies against hnRNP I are implicated in autoimmune diseases.
- Accurate diagnostic markers for systemic sclerosis (SSc) are crucial.
Purpose of the Study:
- To express recombinant hnRNP I protein using a prokaryotic system.
- To investigate the presence of anti-hnRNP I autoantibodies in SSc and other connective tissue diseases (CTDs).
Main Methods:
- Human hnRNP I gene amplified via RT-PCR and cloned into pET-30a vector.
- Recombinant protein expressed in E. coli BL21(DE3).
- Sera from SSc, SLE, SS, MCTD, UCTD, RA patients and controls analyzed by ELISA.
Main Results:
- High expression of recombinant hnRNP I achieved in E. coli.
- Recombinant hnRNP I specifically reacted with SSc patients' sera.
- A higher positive ratio (48.72%) of anti-hnRNP I autoantibodies was observed in SSc patients compared to other autoimmune diseases (AIDs) and controls.
Conclusions:
- Successful prokaryotic expression of human hnRNP I protein.
- Purified hnRNP I protein is a potential diagnostic biomarker for SSc.
- This finding aids in the serological diagnosis of systemic sclerosis.
