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Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
Published on: October 15, 2016
[Screening of anti-Cry1Ac scFv from A phage display antibody library.]
Yun Wang1, Yuan Liu, Xiang Wang
1College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Aim:
To clone human anti-Cry1Ac single-chain antibodies (scFv) from Tomlinson J phage antibody library.
Methods:
A human large phage antibody library was panned for four rounds of "adhesion-elution-amplification". The specificity of the antigen binding activity of the selected clones was identified by ELISA and the variable genes were analyzed and identified.
Results:
After the panning, 2 positive clones were obtained, which could bind Cry1Ac specifically. DNA sequence analysis showed that they had different antibody V region encoding genes.
Conclusion:
By-passing immunization, the specific human anti-Cry1Ac scFv also can be prepared by using phage antibody library.
