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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.

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Related Experiment Video

Updated: Jun 17, 2026

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

Making mixtures to solve structures: structural elucidation via combinatorial synthesis.

Nigel A Lengkeek1, Paul F Greenwood, Blake Nguyen

  • 1Chemistry, School of Biomedical, Biomolecular and Chemical Sciences, The University of Western Australia, Perth, Western Australia 6009, Australia.

Journal of Combinatorial Chemistry
|December 17, 2009
PubMed
Summary
This summary is machine-generated.

A novel domino Horner-Wadsworth-Emmons olefination strategy efficiently synthesizes complex branched hydrocarbons. Gas chromatography-mass spectrometry rapidly identifies geochemically significant alkanes, showcasing combinatorial synthesis for structural elucidation.

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Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
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Last Updated: Jun 17, 2026

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
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Published on: February 6, 2020

Area of Science:

  • Organic Chemistry
  • Geochemistry
  • Synthetic Chemistry

Background:

  • Highly branched alkanes are crucial in geochemistry and petroleum exploration.
  • Efficient synthesis and identification of these compounds remain challenging.

Purpose of the Study:

  • To develop a combinatorial synthesis strategy for homologous series of branched hydrocarbons.
  • To utilize advanced analytical techniques for rapid structural identification.

Main Methods:

  • Domino Horner-Wadsworth-Emmons olefination for constructing polyenones.
  • Combinatorial elaboration to generate diverse hydrocarbon structures.
  • Gas chromatography-mass spectrometry (GC-MS) for mixture analysis and compound identification.

Main Results:

  • Successful preparation of homologous series of (polyen)ones.
  • Generation of corresponding families of highly branched hydrocarbons.
  • Rapid and unambiguous identification of geochemically important highly branched alkanes via GC-MS.

Conclusions:

  • The domino Horner-Wadsworth-Emmons olefination is a powerful tool for synthesizing complex hydrocarbons.
  • Combinatorial synthesis coupled with GC-MS enables efficient structural elucidation.
  • This approach represents the first use of combinatorial synthesis for determining structural connectivity.