Related Experiment Video
Updated: Jun 17, 2026
07:42
Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
Published on: November 26, 2015
DD3(PCA3) gene expression in cancer and prostatic hyperplasia.
E Floriano-Sánchez1, N Cárdenas-Rodríguez, M Castro-Marín
1Departamento de Bioquímica y Biología Molecular, Escuela Médico Militar, México, DF.
Clinical and Investigative Medicine. Medecine Clinique Et Experimentale
|December 17, 2009
Summary
The DD3(PCA3) gene is significantly upregulated in prostate cancer (PCa) tissue compared to benign prostatic hyperplasia (BPH). Measuring DD3(PCA3) gene expression via RT-PCR may aid in early PCa detection.
Area of Science:
- Molecular Biology
- Oncology
- Genetics
Background:
- The DD3(PCA3) gene is recognized for its potential in early cancer detection.
- It is notably upregulated in cancerous cells, particularly in prostate cancer (PCa).
- Its role in distinguishing malignant from benign prostatic conditions warrants investigation.
Purpose of the Study:
- To validate and analyze DD3(PCA3) gene expression in a Mexican population.
- To compare expression levels between intratumoral PCa tissue and benign prostatic hyperplasia (BPH) tissue.
- To assess the utility of real-time Reverse Transcription-Polymerase Chain Reaction (RT-PCR) for this analysis.
Main Methods:
- Analysis of mRNA expression of DD3(PCA3) using RT-PCR.
- Samples from 40 PCa cases and 40 BPH cases were utilized.
- The GAPDH gene was validated as a stable housekeeping gene for normalization.
Main Results:
- DD3(PCA3) gene expression was significantly higher in PCa tissue (1731+/-280 fold) compared to BPH tissue (58.23+/-9.9 fold).
- Expression levels in PCa were approximately 29.74 times greater than in BPH (P < 0.0001).
- GAPDH demonstrated high stability as a reference gene (Pearson correlation of 0.953, P < 0.007).
Conclusions:
- DD3(PCA3) gene expression analysis by RT-PCR shows promise as a diagnostic tool.
- This method could facilitate the early detection of prostate cancer in clinical settings.
- The findings support DD3(PCA3) as a potential biomarker for PCa.
Related Concept Videos
02:23Abnormal Proliferation
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
02:07Tumor Progression
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...