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A multivariate multianalyte screening method for sulfonamides in milk based on front-face fluorescence spectroscopy
N Rodríguez1, M C Ortiz, L A Sarabia
1Department of Chemistry, Faculty of Sciences, University of Burgos, Pza. Misael Bañuelos s/n, 09001 Burgos, Spain.
Analytica Chimica Acta
|December 17, 2009
Summary
A new fluorescence spectroscopy method effectively screens six sulfonamides in milk. This rapid, cost-effective technique meets European standards for food safety testing.
Area of Science:
- Analytical Chemistry
- Food Safety
- Spectroscopy
Background:
- Screening methods must be rapid, cost-effective, and avoid false positives for routine analysis.
- European Regulation 2002/657/EC sets strict criteria for analytical methods in food safety.
- Sulfonamides are a class of antibiotics that require monitoring in milk.
Purpose of the Study:
- To develop and validate a rapid, simple screening method for six sulfonamides in milk.
- To assess the method's performance according to European Union guidelines.
- To ensure reliable detection of sulfonamides to protect public health.
Main Methods:
- Front-face fluorescence emission spectra were modeled using partial least squares class modelling (PLS-CM).
- Milk samples underwent a single derivatization step with fluorescamine.
- The method was validated for analytical sensitivity and probability of false non-compliance.
Main Results:
- The method demonstrated equal analytical sensitivity for all six sulfonamides.
- Multivariate analytical sensitivity was 37.5 µg/L for a mixture of sulfonamides in milk.
- The probability of false non-compliance (alpha) was 0.17 at a threshold value of 0.05.
Conclusions:
- The developed fluorescence spectroscopy method is suitable for screening six sulfonamides in milk.
- The method adheres to the stringent requirements of European Regulation 2002/657/EC.
- This approach offers a reliable and efficient tool for ensuring milk safety.
