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Updated: Jan 24, 2026
07:35
Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions
Published on: July 10, 2021
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Indirect immunofluorescent labeling of viable cells
1Department of Biophysics, Armed Forces Institute of Pathology, Washington, DC, USA.
Methods in Molecular Biology (Clifton, N.J.)
|December 17, 2009
Summary
This study details a standard flow cytometry protocol for indirect antibody staining. The procedure enables cellular analysis and cell sorting using cell surface or intracellular staining of viable cells.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Background:
- Flow cytometry is a powerful technique for analyzing single cells.
- Indirect antibody staining is commonly used to detect specific cellular targets.
- Standardized protocols are crucial for reproducible results in cellular analysis.
Purpose of the Study:
- To describe a recognized flow cytometry procedure for indirect antibody staining.
- To provide a detailed protocol for cell surface and intracellular antibody staining.
- To facilitate cellular analysis and cell sorting of viable cells.
Main Methods:
- The study discusses a standard indirect antibody staining procedure.
- The protocol is designed for flow cytometry applications.
- It covers staining of both cell surface and intracellular targets.
Main Results:
- A recognized flow cytometry procedure for indirect antibody staining is presented.
- The protocol is applicable to cellular analysis and cell sorting.
- Viable cells can be stained for intracellular or cell surface targets.
Conclusions:
- The provided protocol offers a reliable method for indirect antibody staining in flow cytometry.
- This procedure supports accurate cellular analysis and cell sorting.
- The method is suitable for studying cell surface and intracellular markers on viable cells.
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