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Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Labeling Emotion01:20

Labeling Emotion

Emotional labeling is a cognitive process that involves identifying and naming one's emotions, such as anger, fear, happiness, or sadness. It allows individuals to recognize and express their internal emotional states, a critical aspect of emotional regulation and communication. Labeling emotions requires more than mere recognition; it also involves drawing upon memory and contextual cues to understand the current situation and apply a corresponding emotional label. For instance, feeling...
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

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Updated: Jun 17, 2026

Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures
13:50

Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures

Published on: April 3, 2013

Postembedding labeling methods.

Constance Oliver1

  • 1Department of Cell and Molecular Biology, Faculdade de Medicina de Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.

Methods in Molecular Biology (Clifton, N.J.)
|December 17, 2009
PubMed
Summary
This summary is machine-generated.

Postembedding immunogold labeling is a key electron microscopy technique. This chapter details standard and enhanced methods for immunolabeling cell membranes.

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Area of Science:

  • Electron Microscopy
  • Immunocytochemistry
  • Cell Biology

Background:

  • Postembedding immunogold labeling is the predominant immunolabeling method for electron microscopy.
  • This technique involves fixation, embedding, and sectioning of samples prior to immunostaining on grids.

Purpose of the Study:

  • To present the standard protocol for postembedding immunogold labeling.
  • To describe an enhanced method for improving cell membrane labeling.

Main Methods:

  • Direct labeling: Primary antibody conjugated to colloidal gold.
  • Indirect labeling: Unlabeled primary antibody with gold conjugated to secondary antibodies, protein A, or protein G.
  • Colloidal gold-antibody conjugates are utilized, particularly in indirect methods.

Main Results:

  • The chapter provides a comprehensive guide to established postembedding immunogold labeling techniques.
  • An optimized method for enhanced visualization of cell membranes is detailed.

Conclusions:

  • Postembedding immunogold labeling offers versatile approaches for electron microscopy.
  • The described methods facilitate detailed ultrastructural analysis, with specific enhancements for cell membranes.