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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

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Related Experiment Video

Updated: Jun 17, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Computational approaches for microRNA studies: a review.

Li Li1, Jianzhen Xu, Deyin Yang

  • 1Department of Medical Genetics, School of Medicine, Tongji University, Shanghai, 200092, People's Republic of China.

Mammalian Genome : Official Journal of the International Mammalian Genome Society
|December 17, 2009
PubMed
Summary

MicroRNAs (miRNAs) regulate gene expression and are implicated in cancer. Computational methods are crucial for studying miRNA gene finding, target prediction, and regulation, aiding cancer research.

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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Related Experiment Videos

Last Updated: Jun 17, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Genomics

Background:

  • MicroRNAs (miRNAs) are small, endogenous RNAs regulating messenger RNA (mRNA) expression.
  • Aberrant miRNA expression is linked to various cancers, highlighting their role in carcinogenesis.
  • Computational methods are essential for miRNA gene identification and functional analysis.

Purpose of the Study:

  • To review the biological aspects of miRNA genes, including biogenesis and function.
  • To detail computational approaches for miRNA gene finding, target prediction, and regulatory studies.
  • To discuss emerging areas like combinatorial miRNA regulation and non-3'UTR binding sites.

Main Methods:

  • Literature review of miRNA biology and computational studies.
  • Discussion of established and emerging computational methodologies.
  • Identification of online resources for miRNA research.

Main Results:

  • Comprehensive overview of miRNA genomic features, biogenesis, and functional mechanisms.
  • Detailed examination of computational tools for miRNA gene finding and target prediction.
  • Exploration of miRNA gene regulation and novel regulatory mechanisms.

Conclusions:

  • Computational approaches are indispensable for advancing miRNA research in biology and disease.
  • Emerging research directions include complex regulatory networks and alternative binding sites.
  • Online resources facilitate ongoing and future miRNA computational studies.