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Related Experiment Video

Updated: Jun 17, 2026

Oligopeptide Competition Assay for Phosphorylation Site Determination

Oligopeptide Competition Assay for Phosphorylation Site Determination

Published on: May 18, 2017

The intersections between O-GlcNAcylation and phosphorylation: implications for multiple signaling pathways.

Quira Zeidan, Gerald W Hart

Journal of Cell Science

|December 18, 2009

Summary

This summary is machine-generated.

Discover the dynamic interplay between O-linked N-acetylglucosamine (O-GlcNAc) and phosphorylation. This crucial cellular crosstalk regulates signaling pathways and impacts nutrient sensing, stress responses, and neuropathologies.

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Identification of Kinase-substrate Pairs Using High Throughput Screening

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

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Last Updated: Jun 17, 2026

Oligopeptide Competition Assay for Phosphorylation Site Determination

Oligopeptide Competition Assay for Phosphorylation Site Determination

Published on: May 18, 2017

Identification of Kinase-substrate Pairs Using High Throughput Screening

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

Area of Science:

Molecular Biology
Cellular Signaling
Biochemistry

Background:

Nuclear and cytosolic proteins undergo dynamic glycosylation with O-linked beta-N-acetylglucosamine (O-GlcNAc).
O-GlcNAcylation, similar to phosphorylation, occurs on serine/threonine residues and rapidly cycles upon cellular activation.
O-GlcNAc and phosphate exhibit complex crosstalk, affecting each other's regulatory enzymes and signaling pathways.

Purpose of the Study:

To highlight recent findings on the essential crosstalk between phosphorylation and O-GlcNAcylation.
To emphasize the fundamental role of O-GlcNAc in nutrient- and stress-induced signal transduction.
To explore the implications of this crosstalk in cellular processes and neuropathologies.

Main Methods:

Review of recent research findings.
Analysis of protein glycosylation and phosphorylation mechanisms.
Examination of signaling pathway regulation.

Main Results:

O-GlcNAc and phosphate crosstalk occurs in all cellular compartments.
O-GlcNAcylation regulates signaling cascades like NFkappaB and transcription factors.
O-GlcNAc transferase localization and substrate targeting are regulated by insulin signaling.

Conclusions:

Continuous crosstalk between phosphorylation and O-GlcNAcylation is vital for controlling cellular processes.
Understanding this crosstalk is crucial for elucidating mechanisms underlying neuropathologies.
O-GlcNAcylation plays a fundamental role in cellular signal transduction and metabolic regulation.