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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Single Particle Cryo-Electron Microscopy: From Sample to Structure
11:52

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

A toolbox for ab initio 3-D reconstructions in single-particle electron microscopy.

Neil R Voss1, Dmitry Lyumkis, Anchi Cheng

  • 1National Resource for Automated Molecular Microscopy and Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.

Journal of Structural Biology
|December 19, 2009
PubMed
Summary

Researchers developed a new toolbox for 3D structure determination using single-particle electron microscopy. This tool simplifies macromolecular complex reconstruction from 2D images, improving model generation and reliability.

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A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion
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Last Updated: Jun 17, 2026

Single Particle Cryo-Electron Microscopy: From Sample to Structure
11:52

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion
13:43

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

Area of Science:

  • Structural Biology
  • Biophysics
  • Computational Biology

Background:

  • Single-particle electron microscopy (spEM) is crucial for determining macromolecular complex structures.
  • Obtaining reliable 3D reconstructions from 2D images presents a significant challenge in spEM.
  • Existing reconstruction strategies can be complex and difficult to access.

Purpose of the Study:

  • To develop an accessible and user-friendly toolbox for ab initio 3D reconstruction in spEM.
  • To streamline the workflow for calculating 3D volumes from 2D electron microscopy data.
  • To enhance the ease and reliability of generating structural models of macromolecular complexes.

Main Methods:

  • Developed a toolbox integrating multiple ab initio reconstruction techniques.
  • Implemented procedures for random or orthogonal tilt geometry, tomograms, and common lines.
  • Ensured a tightly controlled workflow with transparent data transfer between software packages.

Main Results:

  • Successfully tested the toolbox using the 50S ribosomal subunit.
  • Demonstrated streamlined reconstruction process, reducing bookkeeping needs.
  • Facilitated easier generation of 3D structural models.

Conclusions:

  • The developed toolbox offers multiple accessible ab initio reconstruction algorithms.
  • It simplifies the process of generating 3D volumes from 2D spEM data.
  • Provides a means to evaluate the confidence and reliability of reconstructed structural maps.