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Updated: Jun 17, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Analysis of aptamer sequence activity relationships.

Mark Platt1, William Rowe, Joshua Knowles

  • 1Manchester Interdisciplinary Biocentre, The University of Manchester, UK.

Integrative Biology : Quantitative Biosciences From Nano to Macro
|December 22, 2009
PubMed
Summary
This summary is machine-generated.

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This study optimized aptamer microarray analysis for thrombin binding aptamers (TBAs). Researchers developed a detailed model of DNA quadruplex sequences, improving aptamer selection and detection methods.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Aptamers are DNA sequences with specific binding capabilities.
  • Traditional aptamer analysis uses soluble forms, limiting simultaneous interrogation.
  • On-chip or microarray formats offer high-throughput analysis of aptamer binding.

Purpose of the Study:

  • To calibrate and optimize aptamer microarray systems for high-density analysis.
  • To explore the relationship between aptamer sequence, structure, and binding functionality.
  • To develop a predictive model for aptamer performance on microarrays.

Main Methods:

  • Systematic mutation of known thrombin binding aptamers (TBAs).
  • Utilizing high-density oligonucleotide microarrays for large-scale aptamer screening.

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Primer-Free Aptamer Selection Using A Random DNA Library

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Related Experiment Videos

Last Updated: Jun 17, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
12:23

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

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  • Employing fluorescence-based detection methods to analyze binding affinity and specificity.

    • Main Results:

    • Established experimental conditions and limitations for aptamer microarrays using mutated TBAs.
    • Generated a comprehensive model based on over 40,000 analyses.
    • Identified key structural features influencing quadruplex-forming aptamer functionality.

    Conclusions:

    • Optimized microarray methods enable robust aptamer screening.
    • The developed model enhances understanding of aptamer sequence-function relationships.
    • This work advances aptamer selection for diagnostic and therapeutic applications.