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[Study on purification of total flavones from Trollius ledebouri by macroporous resin]
1Department of Pharmacy, Fuzhou General Hospital of Nanjing Military Region, Fuzhou 350025, China.
Objective:
To study the optimal technologies for the purification of total flavones from Trollius ledebouri with macroporous resin.
Methods:
Static and dynamic adsorption-desorption methods were adopted to choose the optimal type of resin. The adsorption function of D101 macroporous resin and the effects of sample concentration, pH, flow rate and eluant etc. were studied. Then the orthogonal design L9 (3(4)) was used to select the optimum purification process conditions.
Results:
The appropriate technological conditions were as follows: the sample concentration was 0.5 g/mL, pH 6.0, the ratio of sample to D101 macroporous resin was 1:1 (W/W), 4 BV of water was used as purificant and 6 BV of 30% alcohol was used as eluant. Under these conditions, the average content of total flavonoids extract was over 60%.