AtTFC B is involved in control of cell division
Yunlong Du1, Meiqiang Cui, Dan Qian
1Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, The National Plant Gene Research Center, Peking University, Beijing 100871, China.
Abstract:
Tubulin-folding cofactors play important roles in regulating plant development. Arabidopsis tubulin-folding cofactor B (AtTFC B) is an Arabidopsis homolog of mammalian tubulin-folding cofactor B, whose biological function in plant development remains poorly understood. Here we report that the homozygous attfc b (-/-) allele caused embryonic lethality. Embryogenesis was arrested at early embryo stage and the cells contained one or multiple nuclei. Plants carrying a heterozygous attfc b (+/-) allele exhibited enlarged mesophyll cells and leaf epidermal cells with bulged nuclei. Flow cytometry analysis showed increased ploidy in the leaves of the attfc b (+/-) mutant, as well as increased levels of Cdc2A and CycB1;1. In addition, immunofluorescence assay showed increased numbers of spindles and phragmoplasts in the attfc b (+/-) mutant. These results suggest that AtTFC B plays an important role in plant cell division.
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