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shRNA targeting HDGF suppressed cell growth and invasion of squamous cell lung cancer

Jie Meng1, Wei Xie, Liming Cao

  • 1Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, China.

Acta Biochimica Et Biophysica Sinica
|January 1, 2010
PubMed

Insights

Hepatoma-derived growth factor (HDGF) acts as an oncogene in lung cancer. Knocking down HDGF suppressed tumor cell proliferation, migration, and invasion, suggesting it as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Hepatoma-derived growth factor (HDGF) is a nuclear protein implicated in tumorigenesis.
  • HDGF exhibits mitogenic and angiogenic activities, contributing to cancer progression, particularly in non-small cell lung cancer.

Purpose of the Study:

  • To investigate the role of HDGF in lung squamous cell carcinoma.
  • To evaluate the therapeutic potential of targeting HDGF in non-small cell lung cancer.

Main Methods:

  • HDGF expression was suppressed using specific short hairpin RNA (shRNA) delivered via lentivirus in lung squamous cell carcinoma 520 cells.
  • Cell proliferation was assessed using MTT, plate clone assays, and a transplanted tumor model.
  • Cell migration and invasion were evaluated using transwell and Boyden assays.

Main Results:

  • Knockdown of HDGF significantly inhibited cell proliferation both in vitro and in vivo.
  • Suppression of HDGF expression reduced cell migration and invasion capabilities.
  • HDGF was confirmed to promote proliferation, migration, and invasion in lung squamous cell carcinoma.

Conclusions:

  • HDGF functions as an oncogene in the pathogenesis of squamous cell lung cancer.
  • HDGF represents a potential therapeutic target for non-small cell lung cancer treatment.

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