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Updated: Jun 17, 2026
Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice
Published on: September 19, 2016
[Immune response in BALB/c mice immunized with BCG expressing HBV truncated C gene and preS1 gene]
Wen Yin1, Xin Lü, Ying-feng Lei
1Center Laboratory, Fourth Medical Military University, Xi'an 710032, China. yinwen@fmmu.edu.cn
Aim:
To study expressing of HBV truncated core and preS1 protein in BCG and to explore the effect of humoral and cellular immune response stimulated by the recombinant BCG.
Methods:
A shuttle vector was constructed and was transformed into BCG which including truncated Core gene and preS1 gene of HBV. The recombinant BCGs were analyzed with SDS-PAGE and Western blot. The three groups of BALB/c mice were immunized with saline, BCG, BCG-pDE22 and BCG-pDE22-CS1 respectively. Then the antibody titer and CTL effects were evaluated.
Results:
Compared with the control group, the recombinant BCG could express a new protein band of 24 kD which was consistent with the size of CS1 fused protein which displayed a good antigen-binding property by Western blot analysis. The antibody titer significantly increased and CTL effect apparently enhanced in the recombinant BCG immunized group.
Conclusion:
BCG could be as live vector which carrying HBV related genes, which developing a novel vaccine against HBV.
