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Updated: Jun 17, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
Published on: October 28, 2018
Fluorescence enhancement and reflection of the excitation light observed with a multilayered substrate
Takuo Akimoto1, Mitsuru Yasuda
1School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura-machi, Hachiouji, 192-0982 Tokyo, Japan. akimoto@bs.teu.ac.jp
Abstract:
Fluorescence enhancement from a multilayered substrate fabricated with Ag and Al(2)O(3) was investigated using fluorescein, rhodamine B, Cy3, and Cy5 as fluorophores. The change in the fluorescence enhancement with Al(2)O(3) had two peaks and one valley in the range from 0 to 300 nm of Al(2)O(3) thickness, and such peaks and valley were found to appear periodically. Moreover, the reflection of the excitation light from the multilayered substrate was investigated. The reflection of the excitation light periodically changed depending on the Al(2)O(3) thickness as well, and the maximum reflection was observed near the Al(2)O(3) thickness of the peak fluorescence enhancement. It was found that the periodic changes of the fluorescence enhancement and the reflection of the excitation light could be explained, for the most part, with the integral multiples of the lambda/4 derived by a simple interference theory.
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