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Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
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Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants

Published on: May 4, 2018

[Genotypic features of Pseudomonas aeruginosa strains circulating in surgical hospital]

L P Avetisian, M Iu Chernukha, N I Gabrielian

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |January 13, 2010
    PubMed
    Abstract

    Insights

    A dominant clone of Pseudomonas aeruginosa, resistant to multiple antibiotics, circulated in the intensive care unit (ICU) for 15 months. This clone caused hospital-acquired infections in over half of the patients studied.

    Area of Science:

    • Medical Microbiology
    • Infectious Diseases
    • Genetics

    Background:

    • Pseudomonas aeruginosa is a significant cause of hospital-acquired infections, particularly in intensive care units (ICUs).
    • Understanding the genetic diversity and transmission dynamics of circulating strains is crucial for infection control.

    Purpose of the Study:

    • To investigate the genetic diversity of Pseudomonas aeruginosa strains within an ICU.
    • To identify the origin and circulation patterns of epidemic strains.
    • To determine the duration of circulation of a significant clone.

    Main Methods:

    • Genotyping of 106 Pseudomonas aeruginosa isolates using random amplified polymorphic DNA (RAPD) analysis.
    • Isolation of strains from patients, clinical specimens, and fomites.

    Main Results:

    • A single genotype of Pseudomonas aeruginosa dominated, accounting for 72.6% of isolates during the study period.
    • The prevalent genotype was found in 58.3% of patients, indicating intrahospital transmission.
    • The dominant clone exhibited multidrug resistance and was associated with nosocomial infections.

    Conclusions:

    • One multidrug-resistant Pseudomonas aeruginosa clone was prevalent in the ICU for 15 months.
    • Intrahospital transmission of this epidemic strain was confirmed.
    • Continuous molecular-microbiological monitoring is essential for early detection of dangerous hospital strains.

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