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Updated: Jun 17, 2026

Neurogenesis Using P19 Embryonal Carcinoma Cells
Published on: April 27, 2019
[Inhibitory role of SirT7 in the growth of P19 cell line]
Jian-yi Lu1, Ye Zhang, Yu-fei Shen
1National Laboratory of Medical Molecular Biology, Institute of National Laboratory of Medical Molecular Biology, Beijing 100005, China.
Objective:
To study the regulatory role of SirT7, one of class histone deacetylases, on the proliferation of mouse embryonal carcinoma cell line P19.
Methods:
We used an expression plasmid of SirT7 (151-402 amino acid residues) and its vector respectively to establish a stably expressed SirT7 and its control P19 cell lines. Recombinant DNA techniques, Western blot, cell growth curve, and flow cytometry were used in this paper.
Results:
Compared with the control cells, the P19 cells had significantly lower growth rate in stably expressed SirT7. G1 to S cell cycle arrests were only seen in the SirT7 over-expressed cell line.
Conclusion:
SirT7 play a dominant role in the grow inhibition of the P19 cells.
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