Cineholomicroscopy of small animal microcirculation

A holomicrographic system capable of recording the dynamic microscopic events occurring in a living tissue has been developed. This paper discusses the system parameters, the basic configuration, and initial test results. The reconstructed real image from the hologram can be studied with a conventional microscope. The holographic system uses a collecting lens both in recording and in reconstructing. Test results reported include the dissolving of a gas bubble under increased pressure and the movement of polystyrene balls when ejected from a needle. Present resolution is greater than 3 micro, and object velocities greater than 25 mm sec(-1) can be recorded.