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Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
Gene expression patterns distinguish breast carcinomas from normal breast tissues: the Malaysian context
Ivyna Bong Pau Ni1, Zubaidah Zakaria, Rohaizak Muhammad
1Haematology Unit, Cancer Research Centre, Institute for Medical Research, Kuala Lumpur, Malaysia. ivyna@imr.gov.my
Pathology, Research and Practice
|January 26, 2010
Summary
Breast cancer gene expression changes in Malay, Chinese, and Indian populations largely mirror Caucasian patterns. This study identified 33 differentially expressed genes, offering potential breast tumor biomarkers.
Area of Science:
- Genomics
- Molecular Biology
- Oncology
Background:
- Genomic and transcriptomic alterations are key in breast cancer development.
- Breast cancer risk and predisposition show ethnic variations.
Purpose of the Study:
- To identify common transcriptome changes during breast oncogenesis across three ethnic groups: Malays, Chinese, and Indians.
- To compare these changes with existing data from other populations.
Main Methods:
- Gene expression profiling of 43 breast carcinomas and 43 matched normal tissues using Affymetrix U133A GeneChip.
- Analysis of approximately 18,400 transcripts using GeneSpring GX10 software.
- Identification of differentially expressed genes with a >2-fold change at a 99.9% confidence interval (p<0.001).
Main Results:
- Identified 33 significantly differentially expressed genes between normal breast epithelium and carcinoma.
- Key genes identified include CD24, CD36, CD9, TACSTD1, TACSTD2, HBB, LEP, LPL, AKR1C1, AKR1C2, and AKR1C3.
- Gene expression changes in the studied ethnic groups are largely consistent with those observed in the Caucasian population.
Conclusions:
- The majority of gene expression alterations in breast cancer are conserved across the studied ethnic groups and the Caucasian population.
- The identified differentially expressed genes warrant further investigation as potential breast tumor biomarkers.
- These findings may contribute to improved therapeutic and treatment strategies for breast cancer patients.
