Micropropagation of Vanda teres (Roxb.) Lindle

Area of Science:

• Plant Biotechnology
• Horticultural Science
• Orchid Propagation

Background:

• Vanda teres is an orchid species requiring efficient propagation methods.
• Micropropagation techniques are crucial for mass production and conservation of orchids.

Purpose of the Study:

• To establish a high-frequency clonal propagation protocol for Vanda teres.
• To optimize culture media for protocorm-like body (PLB) induction, proliferation, and plantlet regeneration.

Main Methods:

• Nodal segments of Vanda teres were cultured on VW medium with Kinetin and NAA for PLB induction.
• Subsequent culture stages involved media with coconut water, L-glutamine, and banana pulp for shoot and plantlet development.
• Optimized photoperiod and temperature conditions were maintained throughout the process.

Main Results:

• Protocorm-like bodies (PLBs) were induced within 12 weeks and proliferated effectively.
• Regenerated shoots elongated and formed new PLBs, leading to plantlet formation.
• Acclimatized plants bloomed within three years, demonstrating successful whole plant regeneration.

Conclusions:

• The study presents an effective protocol for the rapid clonal propagation of Vanda teres.
• The optimized media and conditions facilitate efficient induction and development of Vanda teres plantlets.
• This method supports the large-scale production and potential conservation of this orchid species.