In vitro propagation through axillary shoot culture of Ranunculus asiaticus L

Area of Science:

• Horticulture
• Plant Biotechnology
• Ornamental Plant Production

Background:

• Ranunculus asiaticus is a key ornamental species in Mediterranean regions.
• Current propagation methods (seed, tuberous root division) have significant limitations.
• Need for efficient propagation of selected Ranunculus genotypes.

Purpose of the Study:

• To present a flowchart for commercial Ranunculus clone production via in vitro axillary budding.
• To highlight the advantages of micropropagation for Ranunculus cultivation.

Main Methods:

• In vitro axillary budding technique.
• Development of a commercial-scale propagation protocol.
• Flowchart for streamlined production.

Main Results:

• Successful micropropagation of Ranunculus clones.
• Production of performant and healthy genotypes.
• Potential for improved cultivation and packaging schedules.

Conclusions:

• In vitro axillary budding is a viable commercial propagation method for Ranunculus.
• Micropropagation offers superior plant quality and production efficiency despite higher initial costs.
• This method supports the supply of advanced genotypes to growers.