Molecularly imprinted polymers for solid-phase extraction of 1-methyladenosine from human urine

Area of Science:

• Analytical Chemistry
• Biomaterials Science
• Chemical Engineering

Background:

• 1-methyladenosine (1-MA) is a urinary biomarker for cancer detection.
• Developing selective methods for 1-MA extraction from complex matrices like urine is challenging.
• Molecularly imprinted polymers (MIPs) offer potential for selective analyte recognition.

Purpose of the Study:

• To synthesize a highly selective MIP for 1-methyladenosine (1-MA).
• To evaluate the MIP's performance as a solid-phase extraction (SPE) sorbent for 1-MA.
• To demonstrate the application of the MIP-SPE method for 1-MA extraction from human urine.

Main Methods:

• A simple MIP synthesis using methacrylic acid as functional monomer and an acetonitrile/water mixture as porogen.
• Batch experiments to assess the MIP's binding characteristics for 1-MA.
• Application of the MIP as an SPE sorbent for selective clean-up and pre-concentration of 1-MA.

Main Results:

• A highly selective MIP for 1-MA was successfully prepared using a straightforward procedure.
• The MIP demonstrated effective binding properties for 1-MA in batch tests.
• The MIP-SPE method successfully extracted and pre-concentrated 1-MA from spiked human urine, removing interfering substances.

Conclusions:

• The developed MIP is a promising sorbent material for selective 1-MA extraction.
• The MIP-SPE method provides an efficient approach for pre-concentrating 1-MA from biological samples.
• This technique facilitates the analysis of 1-MA as a cancer biomarker in urine.