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[Application of the self excision Cre/lox system in plants]
Xiuming Liu1, Xinxin Meng, Haiyan Li
1Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|February 2, 2010
Summary
Marker-free plants are crucial for public acceptance. The Cre/lox site-specific recombination system facilitates efficient self-gene excision in plants, enabling marker-free development.
Area of Science:
- Plant biotechnology
- Molecular biology
- Genetics
Context:
- Public concern regarding genetically modified organisms (GMOs) necessitates the development of marker-free plant varieties.
- Traditional methods often leave selectable marker genes, raising regulatory and public acceptance issues.
- Co-transformation and site-specific recombination are key strategies for achieving marker-free plant development.
Purpose:
- To review the Cre/lox site-specific recombination system and its utility in plant genetic engineering.
- To discuss the application of the Cre/lox system for self-gene excision in plants.
- To provide insights and perspectives on the Cre/lox system based on practical experience.
Summary:
- The Cre/lox system is a powerful tool for site-specific DNA recombination.
- Its application in plants allows for the efficient removal of unwanted DNA elements, such as selectable markers, post-integration.
- This review covers the principles, methods, and successful applications of the Cre/lox system in generating marker-free transgenic plants.
Impact:
- Facilitates the development of marker-free transgenic plants, addressing public concerns and regulatory hurdles.
- Enhances the efficiency and precision of gene transfer and manipulation in plant breeding.
- Offers a versatile platform for complex genetic modifications and genome engineering in various plant species.
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