Related Experiment Video
Updated: Jun 16, 2026

Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays
Published on: March 7, 2025
Effect of Fe deficiency on mitochondrial alternative NAD(P)H dehydrogenases in cucumber roots
Gianpiero Vigani1, Graziano Zocchi
1Dipartimento di Produzione Vegetale, Università degli Studi di Milano, via Celoria 2, 20133 Milano, Italy. gianpiero.vigani@unimi.it
Abstract:
Iron deficiency affects the function of the respiratory chain, primarily at the complex I and complex II levels. Because plant mitochondria possess alternative NAD(P)H dehydrogenases located in the inner membrane, oxidizing NAD(P)H from both cytosol and matrix, we investigated these activities in mitochondria of Fe-deficient roots. External and internal NAD(P)H dehydrogenase activity increased in Fe-deficient mitochondria. Accordingly, NDB1 protein strongly accumulated, while NDA1 did not show differences in Fe-deficient roots. The data presented support, for the first time, the hypothesis that Fe deficiency induces the alternative NAD(P)H dehydrogenases, bypassing the impaired complex I.
Related Concept Videos
Overview of Metabolism
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
Role of Reduced Coenzymes NADH and FADH₂
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...
The Electron Transport Chain
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q in...
C4 Pathway and CAM
C4 Pathway
The C4 pathway is used by plants such as...
Key Elements for Plant Nutrition
