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Updated: Jun 16, 2026
04:56
An Efficient Single-Person Technique for Milk Sampling from Laboratory Mice
Published on: March 28, 2025
Computer controlled 68Ga milking and concentration system
P Gebhardt1, Th Opfermann, H P Saluz
1Leibniz Institute for Natural Product Research and Infection Biology-Hans-Knöll-Institute, Cell and Molecular Biology, Beutenbergstrasse 11a, 07745 Jena, Germany.
Summary
An automated germanium-68/gallium-68 (68Ge/68Ga) generator procedure was developed for reproducible preparation of 68Ga. This method optimizes activity for labeling peptides and proteins for microPET imaging.
Area of Science:
- Radiochemistry
- Nuclear Medicine
- Biotechnology
Background:
- Gallium-68 (68Ga) is a positron-emitting radionuclide crucial for Positron Emission Tomography (PET) imaging.
- Efficient and reproducible preparation of 68Ga is essential for radiopharmaceutical development.
- MicroPET applications require small volumes of highly active 68Ga for labeling biomolecules.
Purpose of the Study:
- To describe an automated milking and concentration procedure for a 68Ge/68Ga generator.
- To optimize the preparation of 68Ga for subsequent radiolabeling.
- To facilitate the use of 68Ga in microPET applications.
Main Methods:
- Development of an automated milking system for the 68Ge/68Ga generator.
- Concentration of eluted 68Ga to small volumes.
- Assessment of 68Ga activity and purity for radiolabeling.
Main Results:
- Successful implementation of an automated 68Ge/68Ga generator milking procedure.
- Reproducible preparation of 68Ga in approximately 200 microliter volumes.
- Optimized 68Ga activity suitable for labeling small amounts of peptides and proteins.
Conclusions:
- The automated procedure ensures reproducible 68Ga preparation.
- The method is ideal for labeling peptides and proteins for microPET imaging.
- This technique enhances the utility of 68Ga in preclinical research and diagnostics.
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